Company DescriptionArtech is the 10th Largest IT Staffing Company in the US, according to Staffing Industry Analysts' 2012 annual report. Artech provides technical expertise to fill gaps in clients' immediate skill-sets availability, deliver emerging technology skill-sets, refresh existing skill base, allow for flexibility in project planning and execution phases, and provide budgeting/financial flexibility by offering contingent labor as a variable cost.Job Description• The purpose of this position is to provide support in evaluating various rodent genetic backgrounds in eliciting robust immune response within GBT. • By performing phenotypic analysis of B cell compartment, deep sequencing of B cell repertoire, and studying antibody responses from immunized rodents, we will be able to understand the utility of these animals in biotherapeutic development. • The incumbent, under the supervision by Pharmaceutical scientist, will be involved in the evaluation of the animals through the use of various immunogens, immunization approaches, and characterization methodSpecific job description: • Perform phenotypic analysis of lymphocyte compartments of rodents• Perform deep sequencing of B cell repertoire of animals and analyze repertoire diversity pre and post immunization• Immunize rodents using various strategies• Characterize antibodies diversity post immunization• Clone and express desired monoclonal antibodies for further analysisExperience: • Knowledge and work experience in the B cell phenotypic analysis by Flow Cytometry• Knowledge and work experience in hybridoma generation• Knowledge and work experience in rodent primary B cell culture• Knowledge and work experience in Ig cloning from B cell• Knowledge and work experience in affinity purification of polyclonal antibodies• Knowledge and work experience in screening and functional assay development for selecting specific monoclonal antibodies• Work experience in NGS of Ig repertoire• Relevant industrial experience is preferredSpecific work plan:a) Perform all aspects of phenotypic analysis of lymphocyte compartments of rodentsb) Perform, design and analyze B cell repertoire of rodents by deep sequencingc) Perform and design immunization strategies and generate hybridomad) Evaluate and characterize immune response and monoclonal antibodies from desired rodentse) Contribute to drafting relevant documentation such as immunization protocols, phenotypic and repertoire analysis reports and laboratory methods.QualificationsBachelor's degree requiredAdditional InformationBest Regards,Anuj Mehta973-967-3402SummaryType: ContractFunction: ResearchExperience level: Mid-Senior LevelIndustry: Pharmaceuticals
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